Use of suppressive subtractive hybridization to identify Flavobacterium columnare DNA sequences not shared with Flavobacterium johnsoniae
نویسندگان
چکیده
منابع مشابه
Development of genetic manipulation in Flavobacterium columnare
Flavobacterium columnare, an important pathogen of freshwater fish, can infect wild, farmed and ornamental fish species worldwide and cause heavy mortality and economic loss. However, reports about protective antigens and virulence factors of F. columnare are rare. Although chondroitin AC lyase has been reported as a probable virulence factor for its degradation of chondroitin sulphates, it has...
متن کاملIdentification of a multidrug efflux pump in Flavobacterium johnsoniae
In this study, the mechanism conferring multiple drug resistance in several strains of flavobacteria isolated from the ovarian fluids of hatchery reared 3-year old brook trout Salvelinus fontinalis was investigated. Metabolic fingerprinting and 16S rRNA gene sequences identified the isolates as Flavobacterium johnsoniae. The isolates exhibited multiple resistances to a wide range of antimicrobi...
متن کاملMutational analysis of the ompA promoter from Flavobacterium johnsoniae.
Sequences that mediate the initiation of transcription in Flavobacterium species are not well known. The majority of identified Flavobacterium promoter elements show homology to those of other members of the phylum Bacteroidetes, but not of proteobacteria, and they function poorly in Escherichia coli. In order to analyze the Flavobacterium promoter structure systematically, we investigated the ...
متن کاملIsolation and characterization of strains of Flavobacterium columnare from Brazil.
Flavobacterium columnare is an important pathogen of freshwater fish, implicated in skin and gill disease, often causing high mortality. An outbreak of skin disease in fingerling and adult Nile tilapia, Oreochromis niloticus (L.), cultivated in a recirculation system, was investigated. Four strains were isolated and characterized by biochemical reactions, enzyme production, fatty acid profile a...
متن کاملGenome sequence of the fish pathogen Flavobacterium columnare ATCC 49512.
Flavobacterium columnare is a Gram-negative, rod-shaped, motile, and highly prevalent fish pathogen causing columnaris disease in freshwater fish worldwide. Here, we present the complete genome sequence of F. columnare strain ATCC 49512.
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2008
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2008.02366.x